Fluorescence Resonance Energy Transfer: a beginner’s guide

April 17th, 2013

FRET, or Fluorescence Resonance Energy Transfer, is a powerful technique dependent on the distance dependent transfer of energy from a donor molecule to an acceptor molecule. FRET can therefore identify molecular interactions by measuring the loss of fluorescence of the donor or gain of fluorescence of the acceptor. It can…

Data - Lateral Flow Immunoassay - InnovaCoat GOLD vs Passive GOLD sensitivity

Maximizing immunoassay performance with smart conjugate design

March 20th, 2013

Labeled antibodies, also known as conjugates, are an integral component in immunoassays. As scientists push the thresholds of detection there is a need to enhance conjugate performance to increase sensitivity. Nobody can turn a bad antibody into a good conjugate, but a great antibody can be ruined if the wrong…


Drug screening assays for phosphate-generating enzymes, ATPase and GTPase assays

January 31st, 2013

This webinar will introduce drug screening assays with a particular focus on phosphate-generating enzymes such at ATPases/GTPases. Topics covered during this twenty minute presentation include: Methods of phosphate detection Application to assays for phosphate generating enzymes Enzyme activity calculations Malachite green assays Improved malachite assays with greater stability/linear range PiColorLock™…

Chart - AbSelect purification flow chart

How to Overcome all your Problems with Secondary Antibodies

December 6th, 2012

This webinar discusses how to overcome problems associated with using secondary antibodies in your immunoassay, including: Secondary antibodies require a series of incubations and wash steps that are both tedious and time consuming Indirect detection can often be a source of non-specific staining within experiments which makes data interpretation difficult…

InnovaCoat GOLD process diagram

Lateral Flow Assay Development and the use of gold nanoparticles

November 1st, 2012

This free webinar brings together three speakers with over 50 years’ experience in assay development, and provides a comprehensive introduction to the development of a lateral flow assay. Starting with the basic principles of antibody selection, reagents and early stage development, the webinar moves on to a discussion on the…

Data - Flow Cytometry

A Beginner’s Guide to Flow Cytometry

September 24th, 2012

This on-demand webinar brings together a unique combination of content from two speakers with more than 50 years of experience in flow cytometry and antibody labeling. Webinar topics include: An outline of the basic principles of flow cytometry, highlighting the underlying simplicity of this powerful technique Key applications based upon…

Western Blot - Lightning-Link HRP table

Western Blotting – The principles and a comparison of indirect vs. direct immunodetection

October 3rd, 2010

Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. Although Western blotting is simple in principle, for a successful outcome, scientists must be aware of the technical caveats which must be overcome. Topics discussed in the webinar include: Sample…