InstantBlue™ ready to use Coomassie protein stain for polyacrylamide gels
InstantBlue™ is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action stains proteins in 15 minutes while leaving a clear background eliminating the need to fix, wash or destain. Formulated for safe use and easy disposal, its ready for use straight out of the bottle and comes in convenient pre-mixed 1 litre volumes. Perfect for use with the InstantBlue™ Stain Tray.
The unique patented mechanism for rapid coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. Some premade and traditional home-made Coomassie R-250 protein stains can take 3 hours or more to fully stain gels.
Why InstantBlue™ Protein Stain?
There’s no need to wash, fix, microwave or destain gels when using InstantBlue™. Homemade stains often require all these steps and most premade solutions require at least one. InstantBlue™ uses a one-step protocol and can be fully destained using standard procedures. The protein staining solution’s acetic acid free formulation doesn’t methylate or acetylate protein during the protein staining protocol, therefore making it fully compatible with mass spectrometry.
Unparalleled sensitivity allows protein levels as low as 5 ng per band to be detected (BSA) when staining overnight. This is thanks to InstantBlue’s protein specific staining which gives a clear background, improving the signal/noise ratio of the stain. Despite the staining power, gels can be left in InstantBlue™ for weeks and still come out with a crystal clear background, all without the need for destaining or protein stain remover.
InstantBlue™ is non-toxic, is sink disposable and thanks to its methanol free composition will not shrink gels. Other stains require solvent disposal procedures and can require microwaving which produces dangerous fumes. The effective staining and safe composition of InstantBlue™ means there’s no need for microwaving, solvent disposal, or fume hoods.
InstantBlue™ will stain at least 40 mini-gels per bottle, boasting economical staining with only 25ml per mini-gel. As a result this allows you to use less protein staining solution when compared to 50-100ml (approximately) of stain per gel for homemade solutions and some pre-mixed solutions.
- Ultra-fast staining – Results in 15 minutes or less.
- One-step procedure – No washing, fixing, microwaving or destaining.
- High sensitivity – 5ng bands detectable*.
- Clear background – High signal/noise ratio.
- Flexible – No over staining.
- Reliable – Quantitative Batch to batch consistency.
- Efficient – Only 25ml per gel.
- Safe composition – Non-toxic. No fume hood or solvent disposal required.
- Methanol free – No gel shrinkage or protein methylation.
- Acetic acid free – No protein aceylation.
- MS Compatible 1 – Destainable. No residual methylation or acetylation.
InstantBlue™ Coomassie Protein Stain Vs Competitors
|InstantBlue™ (Expedeon)||SimplyBlue™ (Invitrogen)||GelCode Blue™ (Pierce)||Home-made|
|No need to wash before staining|
|No need to fix before staining|
|No need to heat or microwave|
|No need to destain|
|No risk of over-staining if left too long|
|Non toxic and easily disposed of|