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Do you have a complex conjugation and haven't been able to find what you need from our range of products? Don't panic! We offer a custom labeling service - just let us know some more details about your requirements and your contact details in the below form and we will get in touch with a solution for you.

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  • ATPase Assay Kit


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Product Range Information

Methods for measuring inorganic phosphate using dyes (especially Malachite Green) are well known, however common problems associated with these reagents include precipitation of the dye and inorganic phosphate complexes, and acidic reagents which lead to non-enzymatic hydrolysis of many phosphorylated substrates.

The Innova Biosciences range of phosphate detection products contains PiColorLock phosphate detection reagent, which changes colour in the presence of inorganic phosphate (Pi) and can therefore measure any enzyme that generates Pi.

PiColorlock has special additives that enhance the stability of dye complexes and ensure low backgrounds with acid-labile substrates, overcoming these common problems.

The reagent itself, along with its Accelerator and Stabiliser are available as a product, for you to utilise within your assay, however we also supply this reagent within our ATPase and GTPase assay kits. These contain all the necessary products for you to measure enzyme activity with the added benefits of the PiColorLock reagent. These also come with utra high quality ATP and GTP, which ensures the lowest possible assay background. 

We also have our PiBind Resin, which has very high affinity for phosphate, meaning it can be used to efficiently clean-up phosphate containing samples, to eliminate contamination of samples during phosphate detection experiments.

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Product Information

The ATPase assay kit is a non-radioactive colorimetric assay kit which utilises a 96-well plate.  It contains all the necessary reagents for measuring enzyme activity and includes PiColorLock™, a phosphate detection reagent, superior to malachite green, which contains special additives that prevent background arising out of non-enzymatic ATP hydrolysis.

Enzyme activity can be determined after 30 minutes of incubation time and the signal can be read for hours due to the highly stability of complexes formed by PiColorLock™.  Therefore the Innova Biosciences ATPase assay kit is ideal for high throughput screening.

The key features of PiColorLock™ within this kit are below:

  • Colorimetric Assay – competitor assays are radioactive
  • Special additive ensures low backgrounds with acid-labile substrates
  • Unparalleled stability of phosphate-dye complexes
  • Reagent is compatible with almost any assay buffer
  • No inhibition of color development by high concentrations of protein
  • Stable reagent formulation - long shelf life
For more information take a look at our guide to enzyme units and assay design or download our phospate detection guide.


In addition to our wide range of kits and products, we also offer services that enable you to get the best out of our technologies. These custom services include specialist conjugations, optimisation, inventory management, bespoke kits and bulk conjugations! 
For more information on these services please click here.

Cost Effectiveness

There are many ways in which Lightning-Link® conjugation methods can save you money over traditional methods. 

Most traditional methods require an in-depth understanding of chemistry, often need expensive equipment and generally result in antibody yields in the range of 50-70%. Our Lightning-Link® kits overcome all of these requirements, providing high quality, consistent conjugates with 100% yield.

The table below shows how the cost of a 1mg antibody – Alkaline phosphatase conjugate using Lightning-Link technologies compares to one produced by traditional methods.


    Traditional Method Lightning-Link® method Notes
Capital equipment     Chromatographic purification system £10,000-£20,000 n/a  
  Fraction collector £1,000-£2,000 n/a  
  Spectrophotometer £1,000-£2,000 n/a  
  Gel filtration media and columns £500 n/a  
Reagent costs Alkaline phosphatase £20 n/a     
Assumes high quality AP as used in LL kits
  Cross-linking reagents and other chemicals £10 n/a  
  Buffers £10 n/a  
  De-salting columns £50 n/a  
  Lightning-Link® kits n/a £255 LL kits at single unit list price
Antibody costs 1mg purified antibody £350 £350 Bulk antibody at £350/mg from commercial source 
  Cost of losses assuming yield of 50% £175 n/a No losses with LL method
Time Staff cost £100 £7 Assumes staff member with salary approx. £30k per annum 
  Overheads £60 £5
Assumes overhead at £10/hour
Total one off costs   £12,590-£24,590 £0  
Total recurring costs   £635 £617  

Recurring costs include antibody, staff and overhead costs, and LL kits at full list price. For commercial applications at large scale LL reagent costs may be negotiated to provide significant cost benefits in association with an appropriate supply and license agreement.

Staff costs do not make any allowance for the fact that the LL procedure may be carried out by a non-graduate technician, whilst a graduate scientist is needed to undertake traditional conjugation methods, with a commensurately higher salary.

Quality Management

Innova Biosciences operate an extensive quality management system and are registered with ISO 9001:2008 (certificate number 54839/A/0001/UK/En).

Our quality system ensures that all of our procedures, ranging from design and development of products, to manufacturing, order processing and fulfilment are fully documented and traceable.  These processes underpin our high standards of product performance and consistency, and provide the formal assurance that our customers need that every product will meet our demanding standards.

Our formal quality system is of particular value to those customers who are working in regulated sectors, such as IVD kit development and sales.  We are very happy to provide appropriate support to customers who are dealing with product registration formalities with authorities such as the FDA or other national regulatory bodies.

Customers who operate their own Quality Systems may wish to undertake a Quality audit of our facilities, and such visits are very welcome.  Please contact us if you need any further information relating to our Quality Systems.

Product Applications

Our labeling and nanoparticle technologies can be used in a variety of applications. For more information, take a look at our products by application summary.



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