Magnetic Resin GST-Tagged GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. After binding of target molecules, the magnetic resin can be readily isolated with the aid of a magnet. The GST tag can be cleaved in bound condition or in eluted condition by specific proteases such as TEV-express and 3C-express. Amintra Glutathione Magnetic is specially designed for magnetic purification of proteins in batch mode. The base matrix is made of cross-linked magnetically charged agarose.Removal of liquid after each step, such as binding, washing and elution can be readily done by pulling the resin down with a magnet.
Amintra's Glutathione resin is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to a high crosslinked agarose matrix that provides a high chemical and physical stability and excellent flow rates. The resin is made in the particle size range of 40-165 ¬µm and has a dynamic binding capacity of 10 mg recombinant GST/ml resin. Amintra Glutathione is stable with all commonly used buffers and reagents including 0.1M NaOH and organic solvents. Removal of the GST tag can be performed whilst the fusion protein is bound to the column or in solution after elution.
Amintra Heparin Resin is an affinity chromatography medium that is used for the purification of biomolecules that show affinity to heparin, such as antithrombin III, coagulation factors and other plasma proteins, DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
Amintra‚Ñ¢ MBP resin is a chromatography medium for purifying recombinant proteins tagged with maltose binding protein (MBP). MBP tagging of recombinant proteins can result in increased expression levels and higher solubility of a target protein, while allowing high purity recovery with a one step purification.
Amintra Affinity resins are part of a range of cost-effective chromatography resins designed for superior purification performance and customer satisfaction. Amintra resins benefit from high binding capacity, excellent specificity & resolution. They have outstanding durability & stability are high flow rate compatible and suitable for all types of chromatography. Amintra resins are available in prepacked columns (1 and 5ml) and as a slurry.
His-tags have been widely employed as a powerful separation approach in the purification of a broad range of proteins and peptides. It is based on the specific interactions between certain transitional metal ions, mostly Cu2+, Ni2+, Zn2+ and Co2+ to the exposed amino acid surface chains containing histidine. The presence of several adjacent histidines such as (His)6-tag increases the affinity to immobilised metal ions. Increasingly, Amintra Ni-NTA and Ni-IDA resins are employed for the purification of histidine-tagged recombinant proteins expressed in bacteria, yeast and mammalian cells.
Magnetic Resin Protein A Protein A is immobilised to highly porous and highly cross-linked agarose incorporating magnetic material. The particles have an open pore structure with excellent mass transfer properties for large protein molecules. Amintra Protein A Magnetic can be used in any commercially available magnetic device or be settled with any magnet (such as a magnet bar or magnetic plate etc.
Antibody Purification Amintra Protein A and Protein G resins are designed on a highly crosslinked sepharose matrix covalently modified with recombinant Protein A and Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with high capacity and affinity for antibodies.
Protein G is immobilised to a highly porous and highly cross-linked magnetic agarose base matrix. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, and can be settled with any magnet. Stored as a 20% slurry in ethanol.
Amintra Protein G resin a fast flow sepharose matrix covalently modified with recombinant Protein G expressed in E. coli. The albumin domain naturally present in these proteins has been deleted to create a resin with a high capacity and affinity for antibodies. Typically binding capacity of 25 mg IgG / ml resin.
Resin Protein L Protein L is an immunoglobulin-binding protein that was expressed, isolated from the bacterial species Peptostreptococcus magnus. Protein L Agarose resin offers a unique ability in a convenient way to separate a wider range of mammalian Immunoglobulin classes and sub-classes compare to other antibody-binding protein as Protein A or G.
For the Fast & Easy Development of Customized Lateral Flow Assays
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Request a pdf copy of our brochure to find out more about our range of antibody and protein labeling technologies.