Thunder-Link® PLUS Oligo Conjugation System

Thunder-Link® PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean-up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods.

Thunder-Link®  PLUS Features and Benefits

  • Quick and easy to use – Save time, no specialist knowledge required
  • High levels of antibody and oligo recovery – Save precious reagents
  • Use your own oligo and antibody, at your desired ratio – Flexible
  • Freeze dried – Ships at ambient temperature, long shelf-life
  • Stringently QC tested – Consistent high quality, excellent batch-to-batch reproducibility
  • Unidirectional chemistry – No risk of cross-linking
  • Covalent bond – Highly stable conjugates
  • Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs – Covers the majority of sequences
  • Linking chemistry works at both 5’ and 3’ end – Provides ability to combine with other modifications
  • Post-conjugation clean-up step – No interference from unbound oligo
  • Positive control antibody and oligo included – Enables confirmation of protocol success
  • Wide range of target proteins – Also applicable to antibody fragments and small proteins

Advantages of Thunder-Link® PLUS:

  • Only 30 minutes antibody and oligo activation
  • Fast oligo conjugation – now only 1 hour!
  • High antibody and oligo recovery
  • Robust and flexible clean-up procedure (now works with antibody fragments and other proteins)

Please do not hesitate to Contact us to find out more!

Thunder-Link® PLUS Kit Components

  1. Thunder-Link® PLUS Oligo Activation reagent
  2. Thunder-Link® PLUS Antibody Activation Reagent
  3. Freeze dried control oligo
  4. Freeze dried control antibody
  5. Separating columns
  6. Thunder-Link® PLUS Wash buffer
  7. Thunder-Link® PLUS Conjugate Clean Up Reagent
  8. Thunder-Link® PLUS Antibody Suspension Buffer
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Immuno-PCR Data

Immuno-PCR data generated using Thunder-Link®. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in unconjugated format from HyTest. The antibody was conjugated to an oligonucleotide using a Thunder-Link® kit, and was used as a detection antibody in a sandwich immuno-PCR assay with a polyclonal anti-CRP antibody as the capture reagent.

The top graph shows a plot of the number of PCR cycles undertaken vs. fluorescence intensity generated by SYBR Green containing PCR probes at particular antigen concentrations. The bottom graph shows this data following conversion to antigen amount vs. cycle number to enable the calculation of a standard curve. The results show that the assay utilizes 1000-fold less capture antibody and 100-fold less detection antibody, and provides 1000-fold greater sensitivity than the equivalent ELISA.

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