Maleimide labels

Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

  • Compatible protein thiolation kit available – no specialist knowledge required
  • Covalent bond – highly stable conjugates
  • Maleimide activated labels can be used with a range of biomolecules, including oligonucleotides – flexible
  • Scalable – easy transfer from R&D to manufacturing
  • Stringently QC tested – consistent high quality, excellent batch-to-batch reproducibility
  • Traditional, well-known conjugation chemistry – trusted method

Supplied as freeze dried formulations, therefore shipping on dry ice isn’t necessary.

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Maleimide activated AP conjugated to Goat anti Rabbit IgG

Figure 1. Thiolated goat anti-rabbit IgG was incubated with maleimide-activated Alkaline Phosphatase. The resulting conjugate was analysed by ELISA using a rabbit IgG coatedplate and p-Nitrophenyl Phosphate (PNPP) as enzyme substrate.

Maleimide activated HRP conjugated to Goat anti Rabbit IgG

Figure 2. Thiolated goat anti-rabbit IgG was incubated with maleimide-activated HRP. The resulting conjugate was analysed by ELISA using a rabbit IgG coated plate and 2-2- Azinobis (3-ethylbenzo-thiazoline-6-sulfonic acid: ABTS) as the HRP enzyme substrate.

 

Maleimide activated Streptavidin conjugated to Goat anti Rabbit IgG

Figure 3. Thiolated goat anti-rabbit IgG was incubated with maleimide-activated Streptavidin. The resulting conjugate was analysed by ELISA using a rabbit IgG coated ELISA plate, biotinylated HRP secondary and the HRP substrate 2-2-Azino-bis (3-ethylbenzo-thiazoline- 6-sulfonic acid) (ABTS).

Maleimide activated RPE conjugated to Goat anti Rabbit IgG

Figure 4. Thiolated goat anti-rabbit IgG was incubated with maleimide-activated R-PE. The resulting conjugate was analysed by ELISA using a rabbit IgG coated plate.

 

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