Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species. Immobilization of protein A creates an affinity resin that can be used to isolate IgG fractions from crude serum, ascites fluid or hybridoma supernatants. Aliquots of Protein A resin may also be used to pull down antibody: antigen complexes in immunoprecipitation experiments. Protein A resin is prepared by coupling highly purified protein A to agarose beads.
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