Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labeling reactions. Successful labeling therefore requires antibodies to be purified prior to labeling. The AbSelect™ Serum Antibody Purification System is a fast and simple method to purify antibodies from these types of media.
AbSelect™ Serum is prepared by coupling highly purified protein A to agarose beads which can be used to purify IgG fractions from ascites fluid and serum. The method involves the capture of the antibody on the AbSelect™ Serum resin and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized.
The components of the The AbSelect™ Serum Antibody Purification System are fully compatible with the Lightning-Link™ antibody labeling kits (available separately), which allows the purified antibody to be immediately labeled with an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of under 30 seconds.
Features and benefits
- Fast and easy to use.
- 100% compatible with the Lightning-Link range of conjugation kits
- All components and buffers supplied
- Purify up to 20mg of antibody from ascites fluid or serum.
- Full technical support available
For more information, please read our Antibody Purification Guide.
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