The AbSelect™ Mouse TCS Purification System enables the selective purification of mouse IgG from hybridoma supernatants without the co-purification of bovine IgG. This is because AbSelect™ Mouse resin has a high affinity and specificity for mouse IgG molecules whereas the binding strength of bovine IgG to AbSelect™ Mouse resin is negligible. In comparison, Protein G does not have the capability to selectively purify mouse IgG from hybridoma supernatants. This is illustrated in the figure below.
The method of AbSelect™ Mouse TCS Purification System involves capture of the antibody on the AbSelect™ Mouse resin and the removal of unwanted substances using a simple wash procedure. The purified antibody is then eluted and neutralized. AbSelect™ Mouse TCS Purification System is not suitable for use with antibodies from other species.
The components of all AbSelect™ kits are fully compatible with the Lightning-Link® and Lightning-Link® Rapid antibody labeling systems (available separately), which allow the purified antibody to be rapidly conjugated to a wide variety of labels.
For more information, please read our Antibody Purification Guide.
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