Antibodies are sometimes only available at low concentrations and often contain low molecular weight substances that interfere in labeling reactions with enzymes, biotin, streptavidin and fluorophores. The AbSelect™ Antibody Concentration and Clean-Up Kit can be used to:
- Quickly and easily concentrate antibodies and proteins
- Remove non-protein additives such as sodium azide, tris and glycine
- Perform a simple buffer exchange for more favourable conjugation conditions
The AbSelect™ Antibody Concentration and Clean-Up Kit method utilizes a simple spin column to easily and quickly remove excess buffer from the antibody thereby providing a more concentrated antibody solution.
Benefits of the AbSelect Concentration and Clean-up kit:
- Easy and quick to use
- High recoveries, around 60-100%
- Suitable for Lightning-Link Ab labeling kits and for other kits
- 3 spin cartidges/collecting tube assemblies
- 1 bottle of AbSelect™ conjugation buffer
The antibody will be retained by the filter, while the buffer can be exchanged by centrifugation into a more suitable one.
Please note that if other proteins (e.g. BSA, Gelatin) are present, they will be retained by the filter as well and concentrated.
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