Lightning-Link® Rapid Dylight® 550

The Expedeon Lightning-Link® Rapid DyLight® 550 antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to DyLight® 550 with 100% recovery of materials. The kit requires only 30 seconds hands-on time and the conjugates will be ready to use under 20 minutes. The technology is fully scalable from 10ug to 1g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.

Lightning-Link_process_diagram

DyLight® 550 emits vibrant orange-to-red fluorescence for a wide array of fluorescence labeling-based applications. It has a strong absorption at 556nm, high fluorescence at 584nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. cm-1M-1) and high quantum yield.

DyLight® dyes are extremely bright and are highly water soluble meaning that a high dye-protein ratio can be achieved without precipitation of the conjugates. Furthermore DyLight® dyes retain their fluorescence over a pH range of 4-9.

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Flow Cytometry data – Rapid DyLight® 550

Mouse anti-human CD3 was conjugated with Dylight® 550 using an Expedeon Rapid Lightning-Link® kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line – negative control; red line – positive staining).

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