Lightning-Link® PE/Atto594

The Expedeon Lightning-Link® PE/Atto594 antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to PE/Atto594 with only 30 seconds hands-on time and with 100% recovery of materials. The technology is fully scalable from 10ug to 1g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.

Lightning-Link_process_diagram

The PE has three maximal absorbance values of 498, 544 and 566nm with the optimal depending on the application. The PE functions as an energy donor for the Atto594 fluorescent dye and transfers its energy via energy resonance transfer. The Atto594 emits the energy received from the PE in the form of long wavelength light at 632nm.

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