Lightning-Link® Horseradish Peroxidase (HRP)

The Expedeon Lightning-Link® Horseradish Peroxidase (HRP) antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides, or any other biomolecule with an available amine group, to Horseradish Peroxidase (HRP) with only 30 seconds hands-on time and with 100% recovery of materials. The technology is fully scalable from 10ug to 1g or more and stringently QC tested for consistent high quality and excellent batch-to-batch reproducibility.

Lightning-Link_process_diagram

Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H202) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.

Please refer to our custom services for bulk orders, get in contact with us to know more about this product or see our full Lightning-Link® range.

Click to read more

Western blot data

Primary antibody directly conjugated to HRP using Lightning-Link

Figure 1. Primary antibody directly conjugated to HRP using Lightning-Link shows significantly enhanced sensitivity in western blotting compared to the traditional indirect technique.

ELISA data

A graph to show the detection absorbance within the Elisa test. Proving that CRP is present and detectable

Figure 2. A mouse monoclonal antibody specific for human CRP (clone C7) was purchased in unconjugated format from HyTest. The unconjugated antibody was linked to HRP using a Lightning Link kit, and was used as detection antibody in a sandwich ELISA using a polyclonal anti-CRP antibody as capture reagent.

 

 

Scalability data

Goat Anti-Rabbit labeled with Lightning-Link® HRP

Figure 3. Goat Anti-Rabbit labeled with Lightning-Link® HRP at 100µg, 1mg, 10mg and 100mg single reaction vials tested with Rabbit IgG in a sandwich ELISA. Detection: ABTS.

Goat anti Rabbit labelled with 4 different batches of Lightning-Link® HRP

Figure 4. Goat anti Rabbit labelled with 4 different batches of Lightning-Link® HRP at 100ug reaction vials tested with Rabbit IgG in a sandwich ELISA. Detection: ABTS.

Click to read more

You may also be interested in