BSA, Ovalbumin or Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen (normally referred to as a hapten) needs to be covalently linked to the carrier protein.
The imm-Link™ immunogen preparation kits (for antigens with sulfhydryl groups) allows sulfhydryl containing haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilized mixture containing the carrier protein and all the required conjugation chemistry.
Upon dissolution of the imm-Link™ mixture proprietary chemicals in the mixture become activated, resulting in the coupling of the hapten to the carrier protein in a gentle and controlled process. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
- Easy to use
- All components supplied
- Wide range of chemistries
- Hands on time to set up conjugation reaction is 20-30 seconds
- Supplied dialysis cartridge ensures conjugates are recovered in high yield
- Full technical support available
Once the conjugation reaction is complete the hapten carrier conjugate is dialysed with ease using the supplied dialysis cartridge to remove unwanted by-products from the conjugation reaction. The hapten conjugate can then be used for the purpose of antibody production. The design of the dialysis cartridge ensures that the hapten conjugate is recovered in high yield.
You might also be interested in our Lightning-Link® antibody labeling kits, which enable the quick labeling of antibodies to a range of labels including enzymes, biotin and streptavidin, and fluorescent dyes and proteins, without any loss of antibody. Find out more here.Click to read more