imm-Link™ immunogen preparation kits for antibody production allow the conjugation of small antigens, often known as haptens, to a carrier protein (such as BSA or Ovalbumin) to maximise an immune response for the generation of antigen reagents.
An antigen is defined as any substance which induces an immune response resulting in the production of antibodies in the host species. The immune response is commonly used to generate antibody (monoclonal and polyclonal) reagents against proteins of interest for use in applications such as Western blotting, immunohistochemistry and flow cytometry. imm-Link™ kits allow the conjugation of small antigens, often known as haptens, to a carrier protein (such as BSA or Ovalbumin) to maximise an immune response for the generation of antigen reagents.
Haptens tend to be poor at generating an immune response when used alone. In order to maximise a response to such molecules they are often linked to larger “carrier” molecules such as Bovine Serum Albumin (BSA), Ovalbumin or Keyhole Limpet Hemocyanin (KLH), using a covalent chemical bond.
The imm-Link™ immunogen preparation kits for antibody production allow haptens to be conjugated to a carrier protein simply by adding a solution of the hapten to a lyophilized mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
|imm-Link™ Immunogen Kit||Target Functional Groups|
|Carboxyl||Glutamic acid, aspartic acid or free carboxyl groups|
|Sulfhydryl||Cysteines or free sulfhydryl groups (SH)|
|Amine||Lysines or free amines (NH2)|
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