PiColorLock™

PiColorLock™ phosphate detection reagent changes colour in the presence of inorganic phosphate (Pi). This can be exploited to measure any enzyme that generates Pi including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins.

The unique formulation of PiColorLock™ affords enhanced assay linearity, dynamic range and colour stability; it is also possible with PiColorLock™ to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.

Features & benefits:

  • Colorimetric assay – Simple, scalable and non-radioactive
  • Compatible with almost any assay buffer – Flexibility over a wide range of existing assays
  • Stable reagent formulation – Long shelf life
  • Unique accelerator – Speeds up color development
  • Unique stabilizer – Suppresses non enzymatic backgrounds with acid-labile substrates (ATP or GTP)
  • PiColorLock™-Pi complex is very stable – No precipitation, results can be measured over several hours – ideal for HTS
  • Wide linear range – No inhibition of color development by high concentrations of protein
  • The unique PiColorLockTM Stabilizer prevents background drift with time

The PiColorLock™ reagent is often used with unstable substrates (e.g. ATP, GTP) that give rise to non-enzymatic background drift with time. The unique stabilizer, provided in the PiColorLock™ phosphate detection system, blocks this non-enzymatic breakdown generating a stable low background. The stable signal can therefore be read up to several hours after the reaction has ended.

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Figure 1. Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color).

Figure 2. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ gives baseline readings.

Figure 3. PiColorLock™ has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

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