Universal Lateral Flow Assay Kit

Universal Lateral Flow Assay Kit

For the Fast & Easy Development of Customized Lateral Flow Assays

The Innova Biosciences Universal Lateral Flow Assay (LFA) Kit is designed to enable the easy development of customized sandwich lateral flow assays. The great advantage of this kit is its adaptability to any pair of capture and detection antibodies, which allows the detection of any type of analyte* without the need to stripe the capture antibody onto LFA strips using expensive equipment.

  • Fully customizable – adaptable to any pair of capture and detection antibodies and can detect any type of analyte*
  • Quick & easy to use – both conjugations can be set up in only 30 seconds
  • No false negative results – assay is compatible with biological samples
  • No specialized or costly equipment
  • Can be qualitatively and quantitatively analyzed using the supplied scoring card or a LFA reader respectively

* The antigen must contain at least two antigenic sites for the binding of the capture and detection antibodies

The kit combines Innova’s easy to use Lightning-Link® antibody labeling and InnovaCoat® GOLD nanoparticle conjugation technologies with an immunochromatographic test performed on Universal-LFA strips. The capture antibody is conjugated to Lightning-Link® Ulfa-Tag, while the detection antibody is conjugated to 40nm InnovaCoat® GOLD, both of which require only 30 seconds to set up.

The capture and detection antibodies are diluted and incubated with the analyte and 40nm InnovaCoat® GOLD-Biotin and then run on Universal LFA strips. Universal LFA strips consist of a nitrocellulose membrane containing a ‘Test line’ (T-line) of immobilized anti- Ulfa-Tag antibody, that binds the Ulfa-Tag-conjugated capture antibody which further binds the analyte in complex with the InnovaCoat GOLD®-detection antibody. A red T-line appears when the analyte is present and the line intensity varies depending on the analyte concentration (see Figure 1). Universal LFA strips also contain a ‘Control-line’ (C-line) striped with streptavidin, which confirms that the test is valid, and an absorbent pad to promote and control the flow of sample through the membrane.

Kit components

3x 100ug Lightning-Link®  Ulfa-Tag conjugation kit

  • Targets amine groups
  • Conjugate up to 3 different capture antibodies
  • Not suitable for nucleic acid or small molecule conjugation
  • Ulfa-Tag is used in many molecular biology applications

3x MINI reaction 40nm InnovaCoat® GOLD

  • Conjugate up to 3 different detection antibodies
  • Conjugate is ready to use in just 20 minutes
  • Forms highly stable conjugates
  • Proprietary surface coating prevents metal-protein interactions

40nm InnovaCoat GOLD®-Biotin

100 Universal-LFA strips

Buffer

2 x 96-well low binding well plates

Scoring card

*T&Cs apply – Universal Lateral Flow Launch: 25% discount only applicable for orders placed directly with Innova Biosciences Ltd. (by emailing orders@innovabiosciences.com, on the phone or via our website). Quote ULFA-Launch2017 when ordering. Does not apply for distributors and re-sellers. Only applies to products and not services. Campaign ends November 30th 2017, 12 noon GMT (7am EST). For more information contact us.

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Universal lateral flow assay kit data

Figure 1. Example of sandwich LFA assay for the detection of CRP in serum using the Universal LFA kit: Lighting-Link® Ulfa-Tag and 40nm IC-GOLD are used to conjugate the capture and detection antibody respectively. Results were analysed using Qiagen ESEQuant reader.

1. Can I use the kit for a competitive assay?

The Universal Lateral Flow Assay kit is not suitable for competitive assays or LFA based on nucleic acids.

2. What should I do if my sample contains biotin?

If your sample contains biotin the signal intensity on the T line won’t be affected; however, the control line may be less intense: we suggest loading more concentrated InnovaCoat-GOLD® biotin (keep the volumes the same) or, if possible, diluting your sample.

3. How can I check that my antibody conjugation reactions are successful?

There is no simple way to test your Ulfa-Tag antibody conjugate, you’ll have to test in your assay varying the concentration suggested in the protocol; you can test your 40nm IC-GOLD conjugate on our Conjugate Check&Go! Kit.

4. Do I need a wash or desalt step?

One of the advantages of the Lighting- Link® technology compared to traditional labeling methods is that conjugate purification is not required. The Lightning-Link® reactions are highly efficient, and the kits have been optimized so that, provided the protocols are followed, there should be only a very low amount of free label left at the end of the conjugation.
We recommend washing the particles adding 10 times the volume of the InnovaCoat® GOLD quencher diluted 1:10 in water to the conjugate (i.e. 1ml 1:10 diluted quencher to 100μl of conjugate) and then centrifuge it in a microfuge at:  9,000g 10 minutes (40,60 and 80nm gold conjugates)

5. How do I measure the particles that are bound to the T line and C line?

This depends on what you want to achieve from the lateral flow assay, and the resources that you have available. A qualitative readout is cost-effective, requiring only a visual assessment, and for this we have provided a score card. A quantitative readout is more expensive, requiring the use of a LFA strip reader. There are plenty of these devices on the market, with the price reflecting the level of sophistication. A LFA strip reader will allow you to create a calibration curve, and will provide reproducible and accurate measurements.

6. What type of linkage to gold is formed?

The antibody becomes covalently and irreversibly attached via lysine residues to the InnovaCoat® surface.

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