Lightning-Link® is an innovative technology that enables direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.
- Require only 30 seconds hands-on time
- Do not require spin or separation steps
- Are easy to use
The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates for either 3 hours (Lightning-Link range) or only 15 min(Lightning-Link Rapid range). Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.
The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.
The technology is fully scalable (µg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.