Oligonucleotide Conjugation Kits
Easy to use Conjugation Kits for Oligonucleotides
Thunder-Link® technology enables quick and easy conjugation of oligonucleotides to antibodies for use in R&D applications, drug discovery and the development of diagnostic kits.
Thunder-Link® Features and Benefits
|Quick and easy to use||Save time, no specialist knowledge required|
|High levels of antibody and oligo recovery||Save precious reagents|
|Customer uses their own oligo and antibody, at their desired ratio||Flexible|
|Freeze dried||Ships at ambient temperature, long shelf-life|
|Stringently QC tested||Consistent high quality, excellent batch-to-batch reproducibility|
|Unidirectional chemistry||No risk of cross-linking|
|Covalent bond||Highly stable conjugates|
|Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs||Covers the majority of sequences relevant for customers|
|Linking chemistry works at both 5’ and 3’ end||Provides ability to combine with other modifications|
|Post-conjugation clean-up step||No interference from unbound oligo|
|Positive control antibody and oligo included||Enables confirmation of protocol success|
Thunder-Link® PLUS allows even faster and more efficient antibody-oligonucleotide conjugation than our original Thunder-Link® kit (discontinued in May 2016). We have developed this new superior kit to enable oligo conjugation in only an hour with higher recovery of materials. Find out more here.
The Thunder-Link® conjugation process
Please do not hesitate to Contact us to find out more!
Custom labeling services
Are you looking to increase assay sensitivity, decrease background or increase stability? Do you require bulk quantities to suit your manufacturing or research needs?
At Innova we can help with all your bioconjugation requirements, so if you can't find what you’re looking for from our range of products, or need something a bit different or at a larger scale, our range of custom services may be of interest to you. These include:
Thunder-Link® PLUS Oligonucleotide Conjugation Kit [Find out more]
|AbPureTM Magnetic Purification Kit [Find out more]|
What buffer should my oligonucleotide be in?
Suitable buffers must be between pH 6 and pH 8. The buffer must not contain primary amine groups. Additives such as BSA and azide should be avoided. Ideally the oligo will be dissolved in a phosphate buffer pH 7.
What size and modifications should my oligo have?
The oligo must be between 20 and 120 base pairs in length and contain a terminal amine group, which must be added during synthesis. All commercial oligo suppliers offer this modification. The efficiency of conjugation is slightly higher with 5’aminated oligos.
How pure should my oligo be, and at what concentration?
The oligo must be HPLC purified and be 60-100µM (high) concentration and be in 100µl of a suitable buffer. If the oligo concentration is greater than 100µM, dilute to 100µM in Wash Buffer.
What buffer should my antibody be in?
Suitable buffers must be between pH 7 and pH 9. The buffer must not contain primary amine groups. Additives such as BSA and azide should be avoided. Ideally the antibody will be dissolved in a phosphate buffer pH 7.
What are the other requirements for my antibody?
The antibody to be activated must be purified and at a concentration of 1mg/ml. Higher antibody concentrations should be diluted to 1mg/ml with wash buffer. With antibody concentrations below 1mg/ml the antibody must be concentrated before use.
How much antibody will the kit conjugate?
The kits are designed to activate 100µg of antibody in 100µl of a suitable buffer.
Conjugation efficiency is about 95-100%.
How do I store my new antibody/oligonucleotide conjugate?
The long term stability of the antibody-oligo conjugate will depend on a multitude of factors, including the antibody and oligo themselves, the storage temperature and conditions.
In order to maximise stability, we would recommend storing the conjugate as concentrated as possible and at a low temperature. We would suggest checking with the antibody and oligo manufacturers if their products can be stored in 50% glycerol at -20°C – most conjugates should be able to be stored in these conditions, as long as the antibody and oligo can handle -20°C. If it is appropriate for your reagents, the addition of preservatives may also be helpful.
What is the difference between Thunder-Link® and Thunder-Link® PLUS?
We have been listening to our Thunder-Link customers since we launched the product and have used their feedback and experiences to develop Thunder-Link® PLUS as a superior alternative to our original Thunder-Link technology. The protocol is much the same only faster; so now antibody and oligo activation only requires 30 minutes and the whole conjugation is finished in an hour compared to overnight as with the original Thunder-Link® technology. There is also an improved antibody and oligo recovery using Thunder-Link® PLUS and the clean-up procedure is more robust and flexible, now working with antibody fragments and small proteins too.
Lou Casta, Ph.D. at Genisphere, LLC.
For more information, take a look at our Immuno-PCR application page.
The detection of rare cells or low concentrations of proteins with accuracy and sensitivity in test samples such as whole blood and serum is a significant problem. This is especially true with standard immunoassays such as ELISA.
With the amplification offered by immuno-PCR, the detection of single nucleic acid molecules is now routine -a DNA probe hybridized to its template offers high sensitivity and specificity. The next generation of immunoassay reagents are being engineered through linking oligonucleotides to a diverse set of molecules for instance antibodies or gold nanoparticles.
Typically oligonucleotide conjugates are used in immuno-PCR which combines the specificity of antibodies with the amplification power of immuno-PCR allowing a 10 to 1000 fold increase in sensitivity. Oligonucleotide conjugates also have the potential to be the platform tool in multiplexed protein diagnostic assays.
Proximity ligation assay
Used to identify proteins co-located within cells.
Proximity extension assay
Variation on ImmunoPCR using two antibodies specific for different epitopes.
Fluorescent antibody labeling
Directly fluorescent oligos or complementary labels.
You can find out more about oligo-antibody conjugates from our recent webinar - Oligo:Ab conjugates - an application guide.