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Thunder-Link® PLUS Oligo Conjugation System

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Thunder-Link® PLUS Oligo Conjugation System

Thunder-Link® PLUS enables simple and rapid conjugation of antibodies to oligonucleotides, with high recovery of materials and a superior clean-up procedure. The kit is quick and simple to use, overcoming time consuming and lengthy protocols associated with standard conjugation methods.

Thunder-Link® Features and Benefits

Features Benefits
Quick and easy to use Save time, no specialist knowledge required
High levels of antibody and oligo recovery Save precious reagents
Use your own oligo and antibody, at your desired ratio Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Unidirectional chemistry No risk of cross-linking
Covalent bond Highly stable conjugates
Suitable for single-stranded oligos of 10-120 bases, double-stranded oligos up to 80 base pairs Covers the majority of sequences
Linking chemistry works at both 5’ and 3’ end Provides ability to combine with other modifications
Post-conjugation clean-up step No interference from unbound oligo
Positive control antibody and oligo included Enables confirmation of protocol success
Wide range of target proteins Also applicable to antibody fragments and small proteins

Advantages of Thunder-Link® PLUS:

  • Only 30 minutes antibody and oligo activation
  • Fast oligo conjugation – now only 1 hour!
  • High antibody and oligo recovery
  • Robust and flexible clean-up procedure (now works with antibody fragments and other proteins)

Please do not hesitate to Contact us to find out more!

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Thunder-Link® PLUS Kit Components

  1. Thunder-Link® PLUS Oligo Activation reagent
  2. Thunder-Link® PLUS Antibody Activation Reagent
  3. Freeze dried control oligo
  4. Freeze dried control antibody
  5. Separating columns
  6. Thunder-Link® PLUS Wash buffer
  7. Thunder-Link® PLUS Conjugate Clean Up Reagent
  8. Thunder-Link® PLUS Antibody Suspension Buffer

 

Related product:

Antibody purification kit compatible with Thunder-Link®

AbPureTM Magnetic Purification Kit [Find out more]

Magnetic purification system

 

   

 

 

Custom labeling services 

Are you looking to increase assay sensitivity, decrease background or increase stability? Do you require bulk quantities to suit your manufacturing or research needs?

At Innova we can help with all your bioconjugation requirements, so if you can't find what you’re looking for from our range of products, or need something a bit different or at a larger scale, our range of custom services may be of interest to you. These include:

Thunder-Link® PLUS MSDSs

Antibody Activation Reagent
Antibody Suspension Buffer
Conjugate Clean-Up Reagent
Control Antibody
Control Oligo
Oligo Activation Reagent

Thunder-Link® PLUS Resources

Antibody – oligonucleotide conjugates are becoming increasingly popular as a tool for biomarker detection.

Typically oligonucleotide conjugates are used in immuno-PCR which combines the specificity of antibodies with the amplification power of immuno-PCR allowing a 10 to 1000 fold increase in sensitivity. Oligonucleotide conjugates also have the potential to be the platform tool in multiplexed protein diagnostic assays.

Take a look below for some more resources on immuno-PCR:

  1. Webinar – Oligo:Antibody Conjugates – an application guide
  2. Immuno-PCR Application page
  3. Immuno-PCR Assay Guide

Got a question?

Applications

Immuno-PCR

ThunderLink Immuno-PCR

For more information, take a look at our Immuno-PCR application page.

The detection of rare cells or low concentrations of proteins with accuracy and sensitivity in test samples such as whole blood and serum is a significant problem. This is especially true with standard immunoassays such as ELISA.

With the amplification offered by immuno-PCR, the detection of single nucleic acid molecules is now routine -a DNA probe hybridized to its template offers high sensitivity and specificity. The next generation of immunoassay reagents are being engineered through linking oligonucleotides to a diverse set of molecules for instance antibodies or gold nanoparticles.

Typically oligonucleotide conjugates are used in immuno-PCR which combines the specificity of antibodies with the amplification power of immuno-PCR allowing a 10 to 1000 fold increase in sensitivity. Oligonucleotide conjugates also have the potential to be the platform tool in multiplexed protein diagnostic assays.

Proximity ligation assay

  ThunderLink ProximityLigationAssay

Used to identify proteins co-located within cells.

Proximity extension assay

  ThunderLink ProximityExtensionAssay

Variation on ImmunoPCR using two antibodies specific for different epitopes.

Fluorescent antibody labeling

Directly fluorescent oligos or complementary labels.

You can find out more about oligo-antibody conjugates from our recent webinar - Oligo:Ab conjugates - an application guide.

 
Buy Online

3 reaction + control (425-0300)

In stock

£551.00

1 reaction + control (425-0000)

In stock

£369.00
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.
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