PiColorLock™ detection reagent

The phosphate detection reagent, PiColorLock™, changes color in the presence of inorganic phosphate (Pi) and this property can be exploited to measure the activity of any enzyme that generates Pi.

The reagent is applicable to a large number of enzymes including ATPases, GTPases, phosphatases, heat-shock proteins and DNA unwinding proteins. The unique formulation of PiColorLock™ affords enhanced assay linearity, dynamic range and color stability; it is also possible with PiColorLock™ to work with unstable substrates (e.g. ATP, GTP) that give high non-enzymatic background signals with other acidic dye-based detection reagents.

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