FAQs

Find answers to frequently asked questions about our product ranges in this section below.

FlexLISA FAQs

1. What is different about the FlexLISA® kit? In a standard ELISA the capture antibody is bound directly to the surface of the well, whereas the 96 stripwell plate provided within the FlexLISA® kit is pre-coated with biotin which binds the capture antibody-streptavidin conjugate with a strong affinity. Therefore, much less capture antibody (40x less)…

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GTP Agarose FAQs

Q1. What is the best buffer to use? There is no ‘right’ answer here. In the absence of any information on the binding requirements of the protein(s) of interest a good starting point is a buffer containing 20mM Hepes, 100-500mM NaCl (or KCl), 20mM MgCl2 and 1mM DTT, pH 7.5. Alternatively, try experimenting with several…

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HRP Check&GO! FAQs

1.What are the best storage conditions for the kit? The kit is shipped at ambient temperature and its components need different storage temperatures. Upon receipt, the plastic pot containing the strips and the HRP detection solution should be stored at 4⁰C. The running buffer and the lyophilized control should be stored at -20⁰C. The lyophilized…

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InnovaCoat® GOLD FAQs

InnovaCoat® GOLD nanoparticles which covalently binds antibodies to form highly stable conjugates

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InstantBlue Protein Stain FAQs

What is the sensitivity of InstantBlue? It can detect as low as 5 ng of protein (BSA). Does InstantBlue has an upper limit of detection? The stain does have an upper limit dependent on the volume of the InstantBlue Stain used. The more InstantBlue, the more Coomassie dye that is free to bind to protein….

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Latex Conjugation Kit FAQs

1. What is different about the Latex Conjugation Kit? The latex particles are specially treated to enhance the handling of the latex and permit easy covalent attachment of antibodies and other proteins. The conjugation reaction is initiated simply by adding a solution of the antibody to the freeze-dried powder; the hands on time is 3…

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Lightning-Link® Kits FAQs

How to conjugate your antibody in one easy step

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Magnetic Conjugation Kit FAQs

Magnetic Conjugation Kit Frequently Asked Questions View below frequently asked questions about Expedeon’ magnetic conjugation kit, if you have further questions please don’t hesitate to get in touch… 1.How many magnetic particles are in each kit? A Mini vial provides 25µl 1% conjugated particles, and a Midi vial provides 250ul 1% conjugate. The amount of…

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PiColorLock Phosphate Detection System FAQs 

Frequently Asked Questions (FAQs) for PiColorLock Phosphate Detection System

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RunBlue Bis-Tris FAQs

Do you know why the gel took 3 hours to run half way? Check whether the appropriate running buffer to separate the proteins is used. Expedeon Bis-tris gels, along with the NuPAGE gels, need MES or MOPS running buffer to separate the proteins. They allow proteins to run slower. Are Expedeon Bis Tris gels larger…

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SunScript FAQs

1. Is SunScript RT compatible with other DNA polymerase to use it in a single-tube reaction? There is no problem to use our SunScript enzyme together with other DNA Polymerase, it should work. In fact, we have a One-Step RT-PCR kit in which we combine SunScript with a High Fidelity Taq Polymerase to perform both…

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Thiol Quantification kit FAQs

When and why would you quantify the number of free thiols in a sample? Quantifying the number of free thiols in a sample can be useful in a number of applications. For example, if the final purpose is the conjugation of a thiolated molecule to a maleimide – activated label, quantifying the number of free…

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