CheckGo banner 1Expedeon Website Banner- ECL Kit  960wBrochure Banner2
Shopping Cart
Your Cart is currently empty.
Select your currency
Search the Innova Biosciences site
Quick Order

High Throughput Colorimetric ATPase Assays (inc. PiColorLock)


High Throughput Colorimetric ATPase Assays

This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock™ reagent and more) and is ideal for high throughput drug screening.

The kits contain PiColorlock, a non-radioactive, superior phosphate detection reagent. 

Key features of PiColorLock™ include:

  • Colorimetric Assay – competitor assays are radioactive.
  • Special additive ensures low backgrounds with acid-labile substrates.
  • Unparalleled stability of phosphate-dye complexes.
  • Reagent is compatible with almost any assay buffer.
  • No inhibition of color development by high concentrations of protein.
  • Stable reagent formulation - long shelf life.

How it works:

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

PiColorLock Phosphate Detection System


Comparison with competitors:

Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see left).

You can purchase PiColorLock™ reagent separately here.

Please do not hesitate to Contact us to find out more!

 contact us button

Components in the 2-plate ATP assay kit (5-plate kit amounts in brackets)

Store at 4°C:

1 x 10ml of PiColorLock™* (1 x 25ml)

1 x 0.25ml of Accelerator (1 x 0.5ml)

1 x 5ml of Stabiliser (1 x 10ml)

1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)

1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)

1 x 5ml of 0.1mM Pi standard (1 x 10ml)

*Take care, the solution is very acidic


Store at -20 or -70oC

4 vials of lyophilized ATP (10 vials)


Store at room temperature

2 x 96-well plates (5 plates)

PiColor-Lock Data

Figure 1. ATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ gives baseline readings.

 PiColorlock data no ALS - new

Figure 2. PiColorLock has also been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.

For more information, please watch our webinar on drug screening-assays for phosphate-generating enzymes below:

I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample

This is almost always due to inadequate mixing of the special stabiliser with the sample and detection reagent. Make sure the stabiliser is pipetted up and down several times to ensure thorough mixing.

I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Phosphate Detection System for this purpose?

Yes, only the phosphate will give a signal; pyrophosphate will not.

I have 5% DMSO in my assay. Can I use PiColorLock?

Yes, the reagent is designed for drug screening work and other situations that require DMSO.

I have phosphate in my enzyme. What can I do?

You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.

Buy Online

192 assays (601-0120)

In stock


480 assays (601-0121)

In stock

Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.

You may also be interested in this/these product(s)

From £66.00