Application notes

Fluorescent detection in lateral flow

October 12th, 2017

Lateral flow assays, also known as immunochromatographic assays or strip tests, are immunoassays which are used to detect the presence, or absence, of a target analyte in a sample. Since they are suitable for point-of-care testing, provide a result extremely quickly, and offer simple, user-friendly operation they are rapidly growing in popularity. To find out…


Immunohistochemistry (IHC) – direct labeling

August 28th, 2017

Lightning-Link® antibody labeling kits can be used to directly conjugate antibodies for IHC to HRP or other labels. The IHC data below shows that it is possible to obtain very good staining whilst avoiding the use of secondary antibodies, therefore saving significantly in time and reagent costs. Anti-human CD20 (clone L26) was conjugated to HRP…


Dye-based detection of inorganic phosphate

August 25th, 2017

Dyes such as Malachite Green are regularly used to detect inorganic phosphate (Pi), an important inorganic ion in a wide variety of biological functions. In the presence of Pi these reagents change color, and a simple absorbance reading can be employed to measure the amount of Pi which has been released from a substrate.  …