Why are resins with two ligand densities offered?
Resins with low levels of ligand substitution may not efficiently retain proteins that have a relatively low affinity for the immobilised ATP, but elution of bound protein from these resins by competing ligands may be easier than from high density resins. Highly substituted resins are more likely to retain low affinity binding proteins during the wash steps, but these resins may require a higher concentration of competing ligand and/or a longer period of incubation for efficient elution. A combi-pack is available with high and low substituted resin, together with a matched control resin, for exploratory work. Resins with low and high ligand density are also available individually and in bulk quantities if required.

The answers to many other questions about Kinasebind™ may be found in the technical brochure.