PiColorLock is a phosphate detection reagent for measuring Phosphotases, ATPases and Nucleotidases.

Colorimetric assays are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.

Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

PiColorlock ALS - Standard Sensitivity
PiColorlock Gold - High Sensitivity

Key benefits:
Available in standard and sensitive format (fig 1).
Long linear range (fig 2).
Special additive ensures low backgrounds with acid-labile substrates (fig 3).
Unparalleled stability of Pi-dye complexes (fig 4).
Reagent is compatible with almost any assay buffer.
No inhibition of color development by high concentrations of protein.
Stable reagent formulation - long shelf life.
Colorimetric, non-radioactive assay