Lightning-Link™ Lightning-Link
is the easiest and quickest method
available for preparing streptavidin
conjugated antibodies (or other
proteins), requiring just 30 seconds
hands-on time, regardless of scale.
Activated streptavidin is covalently
attached in a controlled process
at near-neutral pH. The use of
a stable streptavidin reagent
at a low molar ratio of streptavidin:antibody,
combined with highly efficient
labeling process, means that desalting/dialysis
steps are not needed and that
streptavidin labeling can be carried
out even at microgram scale without
losses.
Streptavidin
Streptavidin
is a 53kDa protein purified from Streptomyces
avidinii, which has widespread applications
in biotechnology due to its very high
affinity to the vitamin biotin. Streptavidin
is a tetrameric molecule, composed of
4 13kDa monomers, each of which can bind
a molecule of biotin.
Click to see a titration
curve of streptavidin-HRP prepared
using Lightning-Link Streptavidin.
Kit
contents:
1 or 3
glass vials of Lightning-Link
Streptavidin mix
