Lightning-Link™ Lightning-Link
is the easiest and quickest method
available for preparing biotinylated
antibodies (proteins), requiring
just 30 seconds hands-on time,
regardless of scale. Biotin with
an extended chain is covalently
attached in a controlled process
at near-neutral pH. The use of
a stable biotin reagent at a low
molar ratio of biotin:antibody,
combined with highly efficient
labeling process, means that desalting/dialysis
steps are not needed and that
biotinylations can be carried
out even at microgram scale without
losses.
Biotin The attachment
of biotin to various chemical sites, called
biotinylation, is an important laboratory
technique, which has been used to study
various processes including protein localization,
protein interactions, DNA transcription
and replication. Biotin binds to the tetrameric
avidin proteins, including streptavidin
and neutravidin, with very high affinity.
This interaction has also been used in
various biotechnological applications,
such as extraction of biotin tagged proteins
and ELISA assays.
Click to see a titration
curve of a typical polyclonal antibody
prepared using Lightning-Link Biotin.
Kit
contents:
1 or 3
glass vials of Lightning-Link
Biotin mix
Modifier
agent
Quencher
reagent
Protocol
Lightning-Link
Biotin Type A and Type B
Lightning-Link Biotin has been optimised for two separate
applications. Type A is intended for assays in which a streptavidin-labeled
detection reagent (eg strep-HRP) will be used, whilst Type
B is optimised for assays in which the biotinylated protein
is captured by streptavidin immobilized on a surface (ie
plates, nitrocellulose, magnetic beads etc).
