The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. The biotin in this kit has an extended linker to facilitate molecular interactions.
Click to see a titration
curve of a typical polyclonal antibody
prepared using Lightning-Link Biotin.
Lightning-Link
Biotin Type A and Type B
Lightning-Link Biotin has been optimised for two separate
applications. Type A is intended for assays in which a streptavidin-labeled
detection reagent (eg strep-HRP) will be used, whilst Type
B is optimised for assays in which the biotinylated protein
is captured by streptavidin immobilized on a surface (ie
plates, nitrocellulose, magnetic beads etc).
