Lightning-Link™ Lightning-Link
is the easiest and quickest method available
for making antibody (protein) conjugates,
requiring just 30 seconds hands-on time.
The antibody is covalently bonded to the
label in a directional and controlled
process at near-neutral pH.
Alkaline
Phosphatase Alkaline phosphatase
(AP) is a hydrolyase enzyme that is frequently
conjugated to antibodies for use in immunoassays.
AP has a lower catalytic rate than HRP,
but has greater stability and maintains
linear reaction kinetics for much longer.
PNPP is a popular colorimetric substrate
for AP; the product of the reaction, p-nitrophenol,
can be read at 405nM. Fluorogenic substrates
(e.g. 4-methylumbelliferyl phosphate;
MUP) may also be employed.
Kit
contents:
1 or 3
glass vials of Lightning-Link
AP mix
Modifier
agent
Quencher
reagent
Protocol
Product Name
Pack Size
Code
Price
Lightning-Link AP conjugation kit
1x100ug AP
702-0005
£60
Lightning-Link AP conjugation kit
3x100ug AP
702-0010
£130
Lightning-Link AP conjugation kit
1mg AP
702-0015
£190
Lightning-Link kits can be
produced to almost any scale. If you need to perform conjugations
on a larger scale than these kits allow, please contact
us at info@innovabiosciences.com.
Innova Biosciences also offers a custom
conjugation service.