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Lightning-Link™ Alkaline Phosphatase
 
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AP kit protocol
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Lightning-Link
Lightning-Link is the easiest and quickest method available for making antibody (protein) conjugates, requiring just 30 seconds hands-on time. The antibody is covalently bonded to the label in a directional and controlled process at near-neutral pH.

Alkaline Phosphatase
Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. AP has a lower catalytic rate than HRP, but has greater stability and maintains linear reaction kinetics for much longer. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed.

 

Kit contents:

  • 1 or 3 glass vials of Lightning-Link AP mix
  • Modifier agent
  • Quencher reagent
  • Protocol
 

 

Product Name
Pack Size
Code
Price
Lightning-Link AP conjugation kit
1x100ug AP
702-0005
£60
Lightning-Link AP conjugation kit
3x100ug AP
702-0010
£130
Lightning-Link AP conjugation kit
1mg AP
702-0015
£190

Lightning-Link kits can be produced to almost any scale. If you need to perform conjugations on a larger scale than these kits allow, please contact us at info@innovabiosciences.com. Innova Biosciences also offers a custom conjugation service.

 

 

 

 

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