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Lightning-Link™ Conjugations
 
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Technical Notes
Lightning-Link conjugations in the presence of BSA
Lightning-Link conjugations in the presence of azide
Lightning-Link conjugations in the presence of gelatin
 
The World's Easiest Conjugation Kits

Seeing is truly believing – why not take a look at the Lightning Link video?  more... 

Direct labeling of antibodies can offer significant benefits in techniques such as flow cytometry, ELISA, western blotting and immunohistochemistry. Without the problems of crossover and/or non-specific binding from secondary reagents it becomes far easier to obtain good quality data.  A reduction in the number of incubation and wash steps also makes assays less tedious and saves time. Unfortunately, there has been no simple way of making directly labeled primary antibodies - until now.

Lightning-Link™ uses a one-step procedure which means that conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale. Separation steps are not required. Recovery of antibody is close to 100%.

Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled manner, creating high quality stable conjugates.

The list of labels available in the one-step Lightning-Link format is shown opposite.

Further technical information is available on the Lightning-Link FAQ page.

If you wish to suggest a label or to receive technology updates and news via our monthly newsletter LightningAlerts please go to our enquiry/feedback form.
 
Lightning-Link™ conjugation technology is protected by worldwide patent filings.

 

Available Kits

Lightning-Link HRP
Lightning-Link AP
Lightning-Link Phycocyanin
Lightning-Link B-PE
Lightning-Link R-PE
Lightning-Link C-PE
Lightning-Link APC
Lightning-Link GOx
Lightning-Link Biotin
Lightning-Link Avidin
Lightning-Link Streptavidin
Lightning-Link CPC
Lightning-Link RPC
Lightning-Link Fluroescein
Lightning-Link Rhodamine
Lightning-Link AMCA
Lightning-Link PerCP
Lightning-Link Cy5
Lightning-Link Cy3
Lightning-Link Cy7
Lightning-Link Cy5.5
Lightning-Link Cy3.5
Lightning-Link Tandem APC/Cy7
Lightning-Link Tandem APC/Cy5.5
Lightning-Link Tandem PE/Cy5
Lightning-Link Tandem PE/Cy7
Lightning-Link Tandem PE/Cy5.5
Lightning-Link Tandem PerCP/Cy5.5
Lightning-Link Atto390
Lightning-Link Atto425
Lightning-Link Atto465
Lightning-Link Atto488
Lightning-Link Atto532
Lightning-Link Atto565
Lightninh-Link Atto590
Lightning-Link Atto594
Lightning-Link Atto610
Lightning-Link Atto611
Lightning-Link Atto620
Lightning-Link Atto633
Lightning-Link Atto637
Lightning-Link Atto655
Lightning-Link Atto680
Lightning-Link Atto700

Coming soon

Lightning-Link Lucifer Yellow
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