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The
Lightning-Link™ conjugation system represents
a quantum leap forward in conjugation
technology. It allows you to make antibody
conjugates with minimal hands-on time
- less than 30 seconds. Lightning-Link™
simplifies immunoassay techniques, such
as western blotting, ELISA and immunohistochemistry,
by eliminating secondary reagents and
cutting the number of incubation and
wash steps.
Despite
its simplicity, Lightning-Link™ is a
very sophisticated conjugation system
in which the antibody is directionally
coupled to the label (and not to itself)
in a controlled fashion, creating high
quality conjugates. As the procedure
is not interrupted by desalting steps,
trial conjugates can be prepared with
microgram quantities of protein and
then scaled up with ease. Lightning-Link™
eliminates problems associated with
scale up; hands-on time is essentially
independent of process scale. Consistency
from batch to batch is easy to achieve,
and recoveries approach 100%. Further
technical information is available on
the Lightning-Link
FAQ page.
The latest
version of the Lightning-Link™
kit brochure is available here.
Lightning-Link
HRP, Lightning-Link
Alkaline Phosphatase , Lightning-Link
Biotin, Lightning-Link
Gox, Lightning-Link
APC, Lightning-Link
B-phycoerythrin and Lightning-Link
R-phycoerythrin kits are currently
available. They will shortly be joined
by kits offering a range of other common
labels (see below). If you'd like to
suggest which labels should be included
in forthcoming Lightning-Link kits or
if you wish to be informed of the availability
of new kits please fill in our simple
customer
feedback form.
Lightning-Link™
conjugation technology is protected
by worldwide patent filings.
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