Direct labeling of antibodies can offer significant benefits in techniques such as flow cytometry, ELISA, western blotting and immunohistochemistry. Without the problems of crossover and/or non-specific binding from secondary reagents it becomes far easier to obtain good quality data. A reduction in the number of incubation and wash steps also makes assays less tedious and saves time. Unfortunately, there has been no simple way of making directly labeled primary antibodies - until now.
Lightning-Link™ uses a one-step procedure which means that conjugates can be prepared with ease. Hands-on time is less than 30 seconds, irrespective of scale. Separation steps are not required. Recovery of antibody is close to 100%.
Despite its simplicity, Lightning-Link™ is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled manner, creating high quality stable conjugates.
The list of labels available in the one-step Lightning-Link format is shown opposite.
Further technical information is available on the Lightning-Link FAQ page.
If you wish to suggest a label or to receive technology updates and news via our monthly newsletter LightningAlerts please go to our enquiry/feedback form.
Lightning-Link™ conjugation technology is protected by worldwide patent filings.
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