Goat-anti-rabbit IgG alkalkine phosphatase conjugate.

Anti-rabbit AP conjugates are widely used in immunochemical procedures such as ELISA, western blotting, and immunocytochemistry.

The goat antiserum used in the preparation of this product is purified on rabbit-IgG agarose to isolate the specific antibody fraction that recognizes rabbit IgG. AP conjugate is prepared by conjugating the affinity purified Goat-anti-rabbit IgG antibody to pure AP enzyme of very high specific activity.

A 1/40,000 dilution of Goat anti-rabbit IgG/HRP conjugate gives an OD of >1.0 after 10 min incubation at 22°C with pNPP substrate.