Goat-anti-mouse IgG alkaline phosphatase conjugate.

Anti-mouse alkaline phosphatase conjugates are widely used in immunochemical procedures such as ELISA, western blotting, and immunocytochemistry.

The goat antiserum used in the preparation of this product is purified on mouse-IgG agarose to isolate the specific antibody fraction that recognizes mouse IgG. AP conjugate is prepared by conjugating the affinity purified Goat-anti-mouse IgG antibody to pure AP enzyme of very high specific activity.

A 1/40,000 dilution of Goat anti-mouse IgG/HRP conjugate gives an OD of >1.0 after 10 min incubation at 22°C with pNPP substrate.