ATP linked to a support via its g-phosphate
has been used for affinity purification of
kinases, heat-shock proteins and other ATP-binding
proteins.
KinaseBind™ is a g-phosphate-linked
ATP resin with a long hydrophillic spacer
that facilitates molecular interactions with
biomolecules. Linkage of ATP via the terminal
phosphate means that the immobilised ligand
cannot be removed by phosphatases in crude
extracts.
