High Throughput Colorimetric ATPase Assays
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.
At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.
Uniquely, Innova Biosciences ATPase assay kits contain highly purified ATP, which have had any free inorganic phosphate removed – this means the assays have the lowest possible background signal.
PiColorLock Phosphate Detection Systems can also be purchased for developing simple assays for any phosphate-generating enzyme.
- Very easy to use
- Ultra-pure substrate
- Low Background
- Colorimetric Assay
- High Linearity
- Stable signal
- Contains all reagents required for the assay
Our shipping times are as follows:
48 hours to the US. 24 to 48 hours to Europe.
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References:
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